Electronic method for autofluorography of macromolecules on two-D matrices. [Patent application]

Patent · Tue Dec 29 23:00:00 EST 1981

OSTI ID:5084824

Davidson, J B; Case, A L

A method for detecting, localizing, and quantifying macromolecules contained in a two-dimensional matrix is provided which employs a television-based position sensitive detection system. A molecule-containing matrix may be produced by conventional means to produce spots of light at the molecule locations which are detected by the television system. The matrix, such as a gel matrix, is exposed to an electronic camera system including an image-intensifier and secondary electron conduction camera capable of light integrating times of many minutes. A light image stored in the form of a charge image on the camera tube target is scanned by conventional television techniques, digitized, and stored in a digital memory. Intensity of any point on the image may be determined from the number at the memory address of the point. The entire image may be displayed on a television monitor for inspection and photographing or individual spots may be analyzed through selected readout of the memory locations. Compared to conventional film exposure methods, the exposure time may be reduced 100 to 1000 times.

• DOE Contract Number: W-7405-ENG-26
• Assignee: Dept. of Energy
• Patent Number(s): None
• Application Number: ON: DE82020861
• OSTI ID: 5084824
• Country of Publication: United States
• Language: English

Related Subjects

440300 -- Miscellaneous Instruments-- (-1989)
47 OTHER INSTRUMENTATION
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BEAMS
BIOMEDICAL RADIOGRAPHY
CAMERAS
COLLOIDS
DIAGNOSTIC TECHNIQUES
DISPERSIONS
ELECTROMAGNETIC RADIATION
ELECTRON BEAMS
FLUOROSCOPY
GELS
IMAGE INTENSIFIERS
IMAGE PROCESSING
LEPTON BEAMS
MATRIX ISOLATION
MEASURING INSTRUMENTS
MEDICINE
MOLECULES
NUCLEAR MEDICINE
PARTICLE BEAMS
POSITION SENSITIVE DETECTORS
PROCESSING
RADIATION DETECTORS
RADIATIONS
RADIOLOGY
TELEVISION CAMERAS
VISIBLE RADIATION