Human cytotrophoblasts acquire aneuploidies as they differentiateto an invasive phenotype

Journal Article · Wed Dec 15 04:00:00 EST 2004 · Developmental Biology

OSTI ID:860787

Weier, Jingly F; Weier, Heinz-Ulrich G; Jung, Christine J; Gormley, Matthew; Zhou, Yuan; Chu, Lisa W; Genbacev, Olga; Wright, AlexiA; Fisher, Susan J

Through an unusual differentiation process, human trophoblast progenitors (cytotrophoblasts) give rise to tumor-like cells that invade the uterus. By an unknown mechanism, invasive cytotrophoblasts exhibit permanent cell cycle withdrawal. Here we report molecular cytogenetic data showing that {approx} 20 to 60 percent of these interphase cells had acquired aneusomies involving chromosomes X, Y, o r16. The incidence positively correlated with gestational age and differentiation to an invasive phenotype. Scoring 12 chromosomes in flow-sorted cytotrophoblasts showed that more than 95 percent of the cells were hyperdiploid. Thus, aneuploidy appears to be an important component of normal placentation, perhaps limiting the proliferative and invasive potential of cytotrophoblasts within the uterus.

• Research Organization: Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
• Sponsoring Organization: USDOE. Office of Management Budget and Evaluation; NationalInstitutes of Health Grants CA88258 and HD30367 and DH44313 and HD41425and HD45736, Department of Defense. Postrate Cancer Research Programfellowship DAMD17-03-1-0157; UCSD Research Evaluation and AllocationCommittee, Howard Hughes Medical Institute Student Research Fellowship57001674
• DOE Contract Number: AC02-05CH11231
• OSTI ID: 860787
• Report Number(s): LBNL--58023; BnR: 400412000
• Journal Information: Developmental Biology, Journal Name: Developmental Biology Vol. 279; ISSN 0012-1606; ISSN DEBIAO
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANEUPLOIDY
CELL CYCLE
CHROMOSOMES
PHENOTYPE
Placenta cytotrophoblast aneuploidy proliferationdifferentiation invasion fluorescence in situ hybridization
UTERUS