Analysis of human invasive cytotrophoblasts demonstrates mosaic aneuploidy

Weier, Jingly F.; Ferlatte, Christy; Baumgartner, Adolf; Nguyen, Ha Nam; Weier, Beatrice A.; Weier, Heinz-Ulrich G.

doi:10.1371/journal.pone.0284317

Analysis of human invasive cytotrophoblasts demonstrates mosaic aneuploidy

Journal Article · Fri Jul 21 00:00:00 EDT 2023 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0284317· OSTI ID:2422609

^[1]; Ferlatte, Christy ^[2]; ^[3]; Nguyen, Ha Nam ^[2]; Weier, Beatrice A. ^[4]; Weier, Heinz-Ulrich G. ^[5]

Univ. of California, San Francisco, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Univ. of California, San Francisco, CA (United States)
Univ. of California, San Francisco, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); York St John University (United Kingdom)
Golden State Dermatology, Walnut Creek, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

A total of 24 chromosome-specific fluorescence in situ hybridization probes for interphase nucleus analysis were developed to determine the chromosomal content of individual human invasive cytotrophoblasts derived from in vitro cultured assays. At least 75% of invasive cytotrophoblasts were hyperdiploid and the total number of chromosomes ranged from 47 to 61. The results also demonstrated that these hyperdiploid invasive cytotrophoblasts showed significant heterogeneity. The most copy number gains were observed for chromosomes 13, 14, 15, 19, 21, and 22 with average copy number greater than 2.3. A parallel study using primary invasive cytotrophoblasts also showed a similar trend of copy number changes. Conclusively, 24-chromosome analysis of human non-proliferating cytotrophoblasts (interphase nuclei) was achieved. Hyperdiploidy and chromosomal heterogeneity without endoduplication in invasive cytotrophoblasts may suggest a selective advantage for invasion and short lifespan during normal placental development.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2422609

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 7 Vol. 18; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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