[3H]Azidodantrolene photoaffinity labeling, synthetic domain peptides and monoclonal antibody reactivity identify the dantrolene binding sequence on RyR1

Journal Article · Fri Jun 14 04:00:00 EDT 2002 · Journal of Biological Chemistry

OSTI ID:838172

Paul-Pletzer, Kalanethee ^[1]; Yamamoto, Takeshi; Bhat, Manju B; Ma, Jianjie; Ikemoto, Noriaki; Jimenez, Leslie S; Morimoto, Hiromi; Williams, Philip G; Parness, Jerome

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1. LBNL Library

Dantrolene is a drug that suppresses intracellular Ca2+ release from sarcoplasmic reticulum in normal skeletal muscle and is used as a therapeutic agent in individuals susceptible to malignant hyperthermia. Though its precise mechanism of action has not been elucidated, we have identified the N-terminal region (amino acids 1-1400) of the skeletal muscle isoform of the ryanodine receptor (RyR1), the primary Ca2+ release channel in sarcoplasmic reticulum, as a molecular target for dantrolene using the photoaffinity analog [3H]azidodantrolene(1). Here, we demonstrate that heterologously expressed RyR1 retains its capacity to be specifically labeled with [3H]azidodantrolene,indicating that muscle specific factors are not required for this ligand-receptor interaction. Synthetic domain peptides of RyR1, previously shown to affect RyR1 function in vitro and in vivo, were exploited as potential drug binding site mimics and used in photoaffinity labeling experiments. Only DP1 and DP1-2, peptide s containing the amino acid sequence corresponding to RyR1 residues 590-609, were specifically labeled by [3H]azidodantrolene. A monoclonal anti-RyR1 antibody which recognizes RyR1 and its 1400 amino acid N-terminal fragment, recognizes DP1 and DP1-2 in both Western blots and immunoprecipitation assays, and specifically inhibits [3H]azidodantrolene photolabeling of RyR1 and its N-terminal fragment in sarcoplasmic reticulum. Our results indicate that synthetic domain peptides can mimic a native, ligand binding conformation in vitro, and that the dantrolene binding site and the epitope for the monoclonal antibody on RyR1 are equivalent and composed of amino-acids 590-609.

• Research Organization: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
• Sponsoring Organization: US Department of Energy; National Institutes of Health Grants 5 R01 AR45593 and AR-16922 and P41 RR01237 and R01 AG-115556, National Science Foundation Grant CHE-9502149; UMDNJ-Robert Wood Johnson Medical School, Petroleum Research Fund, Charles and Johanna Busch Memorial Fund (US)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 838172
• Report Number(s): LBNL--50784
• Journal Information: Journal of Biological Chemistry, Journal Name: Journal of Biological Chemistry Journal Issue: 38 Vol. 277
• Country of Publication: United States
• Language: English

Related Subjects

07 ISOTOPE AND RADIATION SOURCES
08 HYDROGEN
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
CAPACITY
DANTROLENE BINDING TRITIUM
DRUGS
HYPERTHERMIA
IN VITRO
IN VIVO
MUSCLES
PEPTIDES
RESIDUES
SARCOPLASMIC RETICULUM
TARGETS