Molecular cloning and expression of cDNA encoding a lumenal calcium binding glycoprotein from sarcoplasmic reticulum

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.16.6047· OSTI ID:6983904

Leberer, E; Charuk, J H.M.; MacLennan, D H ^[1]; Green, N M ^[2]

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1. Univ. of Toronto, Ontario (Canada)
2. National Institute for Medical Research, London (England)

Antibody screening was used to isolate a cDNA encoding the 160-kDa glycoprotein of rabbit skeletal muscle sarcoplasmic reticulum. The cDNA is identical to that encoding the 53-kDa glycoprotein except that it contains an in-frame insertion of 1,308 nucleotides near its 5{prime} end, apparently resulting from alternative splicing. The protein encoded by the cDNA would contain a 19-residue NH{sub 2}-terminal signal sequence and a 453-residue COOH-terminal sequence identical to the 53-kDa glycoprotein. It would also contain a 436-amino acid insert between these sequences. This insert would be highly acidic, suggesting that it might bind Ca{sup 2+}. The purified 160-kDa glycoprotein and the glycoprotein expressed in COS-1 cells transfected with cDNA encoding the 160-kDa glycoprotein were shown to bind {sup 45}C{sup 2+} in a gel overlay assay. The protein was shown to be located in the lumen of the sarcoplasmic reticulum and to be associated through Ca{sup 2+} with the membrane. The authors propose that this lumenal Ca{sup 2+} binding glycoprotein of the sarcoplasmic reticulum be designated sarcalumenin.

• OSTI ID: 6983904
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:16; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALINE EARTH ISOTOPES
ANIMALS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM 45
CALCIUM ISOTOPES
CELL CONSTITUENTS
CHEMICAL REACTIONS
CLONING
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
DNA-CLONING
ENDOPLASMIC RETICULUM
ENZYMES
EVEN-ODD NUCLEI
GLYCOPROTEINS
HYBRIDIZATION
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHOHYDROLASES
POLYMERIZATION
PROTEINS
RABBITS
RADIOISOTOPES
RECOMBINANT DNA
SARCOPLASMIC RETICULUM
VERTEBRATES