Functional glass slides for in vitro evaluation of interactions between osteosarcoma TE85 cells and mineral-binding ligands

Journal Article · Tue Jul 20 04:00:00 EDT 2004 · Journal of Materials Chemistry

DOI:https://doi.org/10.1039/b408597n· OSTI ID:837413

Song, Jie ^[1]; Chen, Julia; Klapperich, Catherine M; Eng, Vincent; Bertozzi, Carolyn R

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1. LBNL Library

Primary amine-functionalized glass slides obtained through a multi-step plasma treatment were conjugated with anionic amino acids that are frequently found as mineral binding elements in acidic extracellular matrix components of natural bone. The modified glass surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements. Human osteosarcoma TE85 cells were cultured on these functionalized slides and analyses on both protein and gene expression levels were performed to probe the ''biocompatibility'' of the surface ligands. Cell attachment and proliferation on anionic surfaces were either better than or comparable to those of cells cultured on tissue culture polystyrene (TCPS). The modified glass surfaces promoted the expression of osteocalcin, alkaline phosphatase activity and ECM proteins such as fibronectin and vitronectin under differentiation culture conditions. Transcript analysis using gene chip microarrays confirmed that culturing TE85 cells on anionic surfaces did not activate apoptotic pathways. Collectively, these results suggest that the potential mineral-binding anionic ligands examined here do not exert significant adverse effects on the expression of important osteogenic markers of TE85 cells. This work paves the way for the incorporation of these ligands into 3-dimensional artificial bone-like scaffolds.

• Research Organization: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
• Sponsoring Organization: USDOE Director, Office of Science. Office of Basic Energy Sciences. Materials Science and Engineering Division; National Institutes of Health (US)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 837413
• Report Number(s): LBNL--55898
• Journal Information: Journal of Materials Chemistry, Journal Name: Journal of Materials Chemistry Journal Issue: 17 Vol. 14
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
ALKALINE PHOSPHATASE
AMINO ACIDS
EVALUATION
FUNCTIONALS
GENES
GLASS
IN VITRO
OSTEOSARCOMAS
PLASMA
POLYSTYRENE
PROBES
PROLIFERATION
PROTEINS
TISSUE CULTURES
X-RAY PHOTOELECTRON SPECTROSCOPY