Osteogenic differentiation of human dental papilla mesenchymal cells

Journal Article · Fri Apr 21 04:00:00 EDT 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.02.101· OSTI ID:20798908

Ikeda, Etsuko ^[1]; Hirose, Motohiro ^[1]; Kotobuki, Noriko ^[1]; Shimaoka, Hideki ^[2]; Tadokoro, Mika ^[1]; Maeda, Masahiko ^[2]; Hayashi, Yoshiko ^[1]; Kirita, Tadaaki ^[2]; Ohgushi, Hajime ^[1]

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1. National Institute of Advanced Industrial Science and Technology (AIST), Research Institute for Cell Engineering (RICE), Tissue Engineering Research Group, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974 (Japan)
2. Department of Oral and Maxillofacial Surgery, Nara Medical University, 840 Shijocho, Kashihara, Nara 634-8521 (Japan)

We isolated dental papilla from impacted human molar and proliferated adherent fibroblastic cells after collagenase treatment of the papilla. The cells were negative for hematopoietic markers but positive for CD29, CD44, CD90, CD105, and CD166. When the cells were further cultured in the presence of {beta}-glycerophosphate, ascorbic acid, and dexamethasone for 14 days, mineralized areas together with osteogenic differentiation evidenced by high alkaline phosphatase activity and osteocalcin contents were observed. The differentiation was confirmed at both protein and gene expression levels. The cells can also be cryopreserved and, after thawing, could show in vivo bone-forming capability. These results indicate that mesenchymal type cells localize in dental papilla and that the cells can be culture expanded/utilized for bone tissue engineering.

• OSTI ID: 20798908
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 342; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ALKALINE PHOSPHATASE
ASCORBIC ACID
BONE TISSUES
DEXAMETHASONE
GENETIC ENGINEERING
SKELETON
STEM CELLS
TEETH