The BDGP gene disruption project: Single transposon insertions associated with 40 percent of Drosophila genes

Journal Article · Tue Jan 13 04:00:00 EST 2004 · Genetics

DOI:https://doi.org/10.1534/genetics.104.026427· OSTI ID:828564

Bellen, Hugo J ^[1]; Levis, Robert W; Liao, Guochun; He, Yuchun; Carlson, Joseph W; Tsang, Garson; Evans-Holm, Martha; Hiesinger, P Robin; Schulze, Karen L; Rubin, Gerald M; Hoskins, Roger A; Spradling, Allan C

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1. LBNL Library

The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each Drosophila gene by the insertion of a single transposable element. As part of this effort, transposons in more than 30,000 fly strains were localized and analyzed relative to predicted Drosophila gene structures. Approximately 6,300 lines that maximize genomic coverage were selected to be sent to the Bloomington Stock Center for public distribution, bringing the size of the BDGP gene disruption collection to 7,140 lines. It now includes individual lines predicted to disrupt 5,362 of the 13,666 currently annotated Drosophila genes (39 percent). Other lines contain an insertion at least 2 kb from others in the collection and likely mutate additional incompletely annotated or uncharacterized genes and chromosomal regulatory elements. The remaining strains contain insertions likely to disrupt alternative gene promoters or to allow gene mis-expression. The expanded BDGP gene disruption collection provides a public resource that will facilitate the application of Drosophila genetics to diverse biological problems. Finally, the project reveals new insight into how transposons interact with a eukaryotic genome and helps define optimal strategies for using insertional mutagenesis as a genomic tool.

• Research Organization: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
• Sponsoring Organization: National Institutes of Health Drosophila Genome Center Grant GMR PI. Special supplement ACS PI. National Institute of General Medical Sciences; Howard Hughes Medical Institute. Spradling Bellen and Rubin Labs, Baylor College of Medicine LB04-BG0227200 (US)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 828564
• Report Number(s): LBNL--55213
• Journal Information: Genetics, Journal Name: Genetics Journal Issue: 2 Vol. 167; ISSN GENTAE; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DISTRIBUTION
DROSOPHILA
DROSOPHILA TRANSPOSON P-ELEMENT PIGGYBAC MUTAGENESIS
GENES
GENETICS
MUTAGENESIS
PROMOTERS
STRAINS
TRANSPOSONS