Gene disruptions using P transposable elements: An integral component of the Drosophila genome project

Journal Article · Tue Nov 21 04:00:00 EST 1995 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.92.24.10824· OSTI ID:210127

Spradling, A C; Stern, D M ^[1]; Kiss, I ^[2]

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1. Howard Hughes Medical Institute Research Labs., Baltimore, MD (United States)
2. Institute of Genetics, Szeged (Hungary); and others

Biologists require genetic as well as molecular tools to decipher genomic information and ultimately to understand gene function. The Berkeley Drosophila Genome Project is addressing these needs with a massive gene disruption project that uses individual, genetically engineered P transposable elements to target open reading frames throughout the Drosophila genome DNA flanking the insertions is sequenced thereby placing and extensive series of genetic markers on the physical genomic map and associating insertions with specific open reading frames and genes. Insertions from the collection now lie within or near most Drosophila genes, greatly reducing the time required to identify new mutations and analyze gene functions. Information revealed from these studies about P element site specificity is being used to target the remaining open reading frames. 38 refs., 5 figs., 1 tab.

• Sponsoring Organization: USDOE
• OSTI ID: 210127
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 24 Vol. 92; ISSN PNASA6; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA SEQUENCING
DROSOPHILA
EVALUATION
GENETIC MAPPING