In situ quantification of genomic instability in breast cancer progression

Conference · Thu May 15 04:00:00 EDT 2003

OSTI ID:823070

Ortiz de Solorzano, Carlos ^[1]; Chin, Koei; Gray, Joe W; Lockett, Stephen J

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1. LBNL Library

Genomic instability is a hallmark of breast and other solid cancers. Presumably caused by critical telomere reduction, GI is responsible for providing the genetic diversity required in the multi-step progression of the disease. We have used multicolor fluorescence in situ hybridization and 3D image analysis to quantify genomic instability cell-by-cell in thick, intact tissue sections of normal breast epithelium, preneoplastic lesions (usual ductal hyperplasia), ductal carcinona is situ or invasive carcinoma of the breast. Our in situ-cell by cell-analysis of genomic instability shows an important increase of genomic instability in the transition from hyperplasia to in situ carcinoma, followed by a reduction of instability in invasive carcinoma. This pattern suggests that the transition from hyperplasia to in situ carcinoma corresponds to telomere crisis and invasive carcinoma is a consequence of telomerase reactivation afertelomere crisis.

• Research Organization: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
• Sponsoring Organization: USDOE Director. Office of Energy Research. Office of Health and Environmental Research; NIH contract N01-CO-56000. NIH grants CA67412 and CA58207, DOD grants DAM D17-00-1-0227 and DAMD17-00-1-0306; Carl Zeiss, Inc. (US)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 823070
• Report Number(s): LBNL--52688
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
BIOLOGY
CARCINOMAS
EPITHELIUM
FLUORESCENCE
GENETICS
IMAGE SEGMENTATION GENOMIC INSTABILITY TELOMERE CRISIS FISH
IN-SITU HYBRIDIZATION
INSTABILITY
MAMMARY GLANDS
MEDICINE
NEOPLASMS
TELOMERES