In situ quantification of genomic instability in breast cancer progression
- LBNL Library
Genomic instability is a hallmark of breast and other solid cancers. Presumably caused by critical telomere reduction, GI is responsible for providing the genetic diversity required in the multi-step progression of the disease. We have used multicolor fluorescence in situ hybridization and 3D image analysis to quantify genomic instability cell-by-cell in thick, intact tissue sections of normal breast epithelium, preneoplastic lesions (usual ductal hyperplasia), ductal carcinona is situ or invasive carcinoma of the breast. Our in situ-cell by cell-analysis of genomic instability shows an important increase of genomic instability in the transition from hyperplasia to in situ carcinoma, followed by a reduction of instability in invasive carcinoma. This pattern suggests that the transition from hyperplasia to in situ carcinoma corresponds to telomere crisis and invasive carcinoma is a consequence of telomerase reactivation afertelomere crisis.
- Research Organization:
- Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
- Sponsoring Organization:
- USDOE Director. Office of Energy Research. Office of Health and Environmental Research; NIH contract N01-CO-56000. NIH grants CA67412 and CA58207, DOD grants DAM D17-00-1-0227 and DAMD17-00-1-0306; Carl Zeiss, Inc. (US)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 823070
- Report Number(s):
- LBNL--52688
- Country of Publication:
- United States
- Language:
- English
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