Towards a full karyotype screening of interphase cells: 'FISH and chip' technology
Numerical chromosome aberrations are incompatible with normal human development. Our laboratories develop hybridization based screening tools that generate a maximum of cytogenetic information for each polar body or blastomere analyzed. The methods are developed considering that the abnormality might require preparation of case-specific probes and that only one or two cells will be available for diagnosis, most of which might be in the interphase stage. Further more, assay efficiencies have to be high, since there is typically not enough time to repeat an experiment or reconfirm a result prior to fertilization or embryo transfer. Structural alterations are delineated with break point-spanning probes. When screening for numerical abnormalities, we apply a Spectral Imaging-based approach to simultaneously score as many as ten different chromosome types in individual inter phase cells. Finally, DNA micro-arrays are under development to score all of the human chromosomes in a single experiment and to increase the resolution with which micro-deletions can be delineated.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- Cancer Research Foundation of America; US Department of Defense, US Army Medical Research and Material Command (BC980937); University of California (U.C.) Systemwide Biotechnology Research and Education Program, Director, Office of Science, Office of Biological and Environmental Research (US)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 815382
- Report Number(s):
- LBNL-52936; MCEND6; R&D Project: 864A1A; TRN: US200319%%193
- Journal Information:
- Molecular and Cellular Endocrinology, Vol. 183, Issue Supplement 1; Other Information: Journal Publication Date: October 22, 2001; PBD: 23 Jun 2003; ISSN 0303-7207
- Country of Publication:
- United States
- Language:
- English
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