Metabolism of 7-nitrogenz[a]anthracene by intestinal microflora
- National Center for Toxicological Research, Jefferson, AR (United States)
Pure cultures of anaerobic intestinal bacteria and mixed fecal microflora from human, rat, mouse, and pig were screened for the ability to metabolize 7-nitrobenz[a]anthracene. Based on analysis by high-performance liquid chromatography (HPLC) and by ultraviolent (UV), mass, and nuclear magnetic resonance (NMR) spectral techniques, the compounds were identified as 7-aminobenz[a]anthracene and benz[a]anthracene 7,12-dione. Identification of 7-NH{sub 2}BA as a metabolite of 7-NO{sub 2}BA indicates that the anaerobic intestinal bacteria are capable of reducing 7-NO{sub 2}BA to potentially bioactive intermediates. The reductive capacities of the mixed intestinal microflora were generally greater than those of pure cultures. Thus, metabolism of 7-NO{sub 2}BA in the intestinal tract may be underestimated if pure cultures are used as the sole method for evaluating the potential hazard.
- OSTI ID:
- 81230
- Journal Information:
- Journal of Toxicology and Environmental Health, Vol. 43, Issue 3; Other Information: PBD: 1994
- Country of Publication:
- United States
- Language:
- English
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