Metabolism of benzo(a)pyrene and 7,12-dimethylbenz(a)anthracene in cultured human fetal aortic smooth muscle cells

Journal Article · Mon Jul 30 04:00:00 EDT 1979 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(79)90574-5· OSTI ID:6783511

Bond, J A; Kocan, R M; Benditt, E P; Juchau, M R

Cultured human fetal aortic smooth muscle cells derived from the abdominal aorta converted benzo(a)pyrene (BaP) and 7,12-dimethylbenz(a)anthracene (DMBA) via cytochrome P-450-dependent monooxygenation to metabolites detectable by both a highly sensitive radiometric assay and high pressure liquid chromatography (HPLC). Cells incubated with /sup 3/H-BaP transformed this substrate primarily to phenols. /sup 14/C-DMBA was converted to metabolites that cochromatographed with 12-hydroxymethyl-methylbenz(a)anthracene, 7-hydroxymethyl-12-methylbenz(a)anthracene, 7- 7,12-dihydroxymethylbenz(a)anthracene, and trans-8,9-dihydrodiol-7,12-DMBA. Exposure of cells in culture to 13 ..mu..M 1,2-benz(a)anthracene resulted in increased oxidative metabolism of both BaP and DMBA. In the case of BaP, total phenol formation was increased, while with DMBA all metabolites detected by HPLC were increased. Support for the potential role of metabolism of polycyclic aromatic hydrocarbons by aortic smooth muscle cells in the etiology of atherosclerosis was obtained.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 6783511
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 25:5; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AORTA
AROMATICS
ARTERIES
BENZOPYRENE
BIOCHEMICAL REACTION KINETICS
BLOOD VESSELS
BODY
CARBON 14 COMPOUNDS
CARDIOVASCULAR SYSTEM
CELL CULTURES
CHROMATOGRAPHY
CONDENSED AROMATICS
COUNTING TECHNIQUES
CYTOCHROMES
DIMETHYLBENZANTHRACENE
ENZYME ACTIVITY
FETUSES
HYDROCARBONS
KINETICS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
LIQUID SCINTILLATORS
METABOLISM
METABOLITES
MUSCLES
ORGANIC COMPOUNDS
ORGANS
PHOSPHORS
PIGMENTS
RADIOASSAY
REACTION KINETICS
REDOX PROCESS
REPROCESSING
SCINTILLATION COUNTING
SENSITIVITY
SEPARATION PROCESSES
TISSUES
TRITIUM COMPOUNDS