Ultrahigh-Resolution Colocalization of Spectrally Separable Point-like Fluorescent Probes
Journal Article
·
· Methods
OSTI ID:803754
- LBNL Library
An ultrahigh-resolution colocalization method based on the simultaneous acquisition and analysis of spectrally separated images of the excitation point-spread function of point-like fluorescent probes is reviewed. It is shown that molecular distances can be measured with accuracy better than 10 nm using conventional far-field optics. A detailed account of the methodology, theoretical considerations, signal processing, and data fitting algorithms is given.
- Research Organization:
- Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
- Sponsoring Organization:
- USDOE Director, Office of Science; National Institutes of Health (US)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 803754
- Report Number(s):
- LBNL--47257; B& R 400412000
- Journal Information:
- Methods, Journal Name: Methods Journal Issue: 1 Vol. 25
- Country of Publication:
- United States
- Language:
- English
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