Ultrahigh resolution multicolor colocalization of single fluorescent probes

Weiss, Shimon; Michalet, Xavier; Lacoste, Thilo D.

Ultrahigh resolution multicolor colocalization of single fluorescent probes

Patent · Tue Jan 18 04:00:00 EST 2005

OSTI ID:1175204

Weiss, Shimon; Michalet, Xavier; Lacoste, Thilo D.

A novel optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described. Two unique families of fluorophores are used, namely energy-transfer fluorescent beads and semiconductor nanocrystal (NC) quantum dots, that can be excited by a single laser wavelength but emit at different wavelengths. A novel multicolor sample-scanning confocal microscope was constructed which allows one to image each fluorescent light emitter, free of chromatic aberrations, by scanning the sample with nanometer scale steps using a piezo-scanner. The resulting spots are accurately localized by fitting them to the known shape of the excitation point-spread-function of the microscope.

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Research Organization:: The Regents of the University of California, Oakland, CA (United States)

Sponsoring Organization:: USDOE

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 6,844,150

Application Number:: 09/925,100

OSTI ID:: 1175204

Country of Publication:: United States

Language:: English

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