Optimal conditions and specificity of interaction of a distinct class of nonhistone chromosomal proteins with DNA

Journal Article · Mon Apr 05 23:00:00 EST 1976 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00652a019· OSTI ID:7366113

Thomas, T L; Patel, G L

A subclass of nonhistone chromatin proteins with high DNA affinity has been isolated from rat liver. The interaction of the isolated proteins with DNA in vitro was characterized utilizing a nitrocellulose filter binding technique. The temperature, time, concentration, ionic strength, and pH dependence were characterized. Optimal interaction was observed at 0.19 M NaCl, pH 7.5, with a protein to DNA ratio of 13 (w/w). Equilibrium and kinetic competition experiments indicated that these proteins interact optimally with A-T rich and single-stranded DNA. The data also suggest that these proteins might affect the helix-coil transition of DNA.

• Research Organization: Univ. of Georgia, Athens
• OSTI ID: 7366113
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 15:7; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CHROMATIN
DIGESTIVE SYSTEM
DNA
FILTERS
GLANDS
KINETICS
LIVER
MAMMALS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PH VALUE
PROTEINS
RATS
REACTION KINETICS
RODENTS
TEMPERATURE DEPENDENCE
VERTEBRATES