DNA cytophotometry of chromosomes in a case of chronic myelogenous leukemia

Journal Article · Tue Jan 01 04:00:00 EST 1974 · Clin. Chem.; (United States)

OSTI ID:7360002

Mayall, B H; Carrano, A V; Rowley, J D

The DNA-stain content of human metaphase chromosomes is measured by use of quantitative cytochemistry, scanning cytophotometry, and image-processing techniques. This approach is applied to chromosomes from a female with chronic myelogenous leukemia. Measurements are normalized for each cell and expressed on a scale by which the sum of the measurements for the 44 autosomal chromosomes is 100 units; the normalized measurements are compared with standard values from eight normal persons. Banding analysis of the leukemic cells shows a 9q+/22q- translocation. This is confirmed by the DNA measurements; 0.42 units are involved. In addition, measurements of both leukemic and nonleukemic cells reveal a 9+/20- translocation that cannot be detected visually; 0.12 units are involved. Neither translocation shows a net loss of DNA; any loss exceeding 0.05 units (10 x 10/sup 6/ base pairs of DNA) would be significant (p < 0.05). These results illustrate the power of this approach and bear on the etiology of chronic myelogenous leukemia.

• Research Organization: Univ. of California, Livermore
• OSTI ID: 7360002
• Journal Information: Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 20:8; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
550600* -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BIOLOGY
CHROMOSOMES
CYTOLOGY
DISEASES
DNA
FLUORESCENCE
GENETICS
HEMIC DISEASES
LEUKEMIA
LUMINESCENCE
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOTOGRAPHY
TRANSLOCATION