Quantification by DNA-based cytophotometry of the 9q + /22q-chromosomal translocation associated with chronic myelogenous leukemia

Journal Article · Sat Oct 01 04:00:00 EDT 1977 · Cancer Res.; (United States)

OSTI ID:5679941

Mayall, B H; Carrano, A V; Moore, II, D H; Rowley, J D

DNA-based cytophotometry was used to analyze metaphase chromosomes in four patients with chronic myelogenous leukemia. In three of these patients, both Philadelphia chromosome (Ph/sup 1/)-positive and Ph/sup 1/-negative cells were measured. On the basis of these three patients, the characteristic 9q + /22q- translocation of chronic myelogenous leukemia involves the net transfer of 0.325% of the autosomal genome; there is no evidence of net gain or loss of DNA (apart from duplication of the Ph/sup 1/ chromosome in one patient), and no significant difference is found in the amount of DNA transferred in different patients. Significant differences are found among patients in the derived Chromosomes 9 and the Ph/sup 1/ chromosomes and are ascribed to preexisting variations in the Ph/sup 1/-negative cells of these patients. There is no evidence in these patients of any further cytogenetic lesion associated with chronic myelogenous leukemia.

• Research Organization: Univ. of California, Livermore
• OSTI ID: 5679941
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 37; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
BIOLOGY
CELL DIVISION
CHEMICAL ANALYSIS
CHROMOSOMAL ABERRATIONS
CYTOLOGY
DISEASES
DNA
HEMIC DISEASES
LEUKEMIA
MITOSIS
MUTATIONS
MYELOID LEUKEMIA
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
QUANTITATIVE CHEMICAL ANALYSIS
TRANSLOCATION