Alterations in RNA turnover following x-irradiation. [Mouse embryonic fibroblasts]

Journal Article · Wed Jan 01 04:00:00 EST 1975 · Radiat. Res.; (United States)

OSTI ID:7353043

Hetzel, F W; Kolodny, G M

Mouse embryonic fibroblasts, 3T3, were irradiated in growing and confluent states with 280KV x-rays to a dose of 3750 rads. All cells were prelabeled while growing with /sup 14/C thymidine for 24 hours and for 24 hours with /sup 3/H uridine in either the growing or confluent state. The turnover of prelabeled cellular RNA was then studied. While RNA turnover normally occurs in confluent cells and does not occur in subconfluent cells the results of this study suggested the existence of RNA turnover in both cell populations following radiation. The rate of RNA turnover in irradiated confluent cells was less than that seen in normal confluent cells while for irradiated growing cells the rate of turnover was higher than in controls. The two rates were approximately equal in both irradiated populations. In each case the amount of prelabeled DNA lost from the acid insoluble fraction was approximately 50 percent after 10 days. The possible significance of these results in relation to radiation induced giant cell formation and alterations in RNA minor bases will be discussed.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 7353043
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 62; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
EMBRYOS
FIBROBLASTS
IONIZING RADIATIONS
MAMMALS
METABOLISM
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RNA
RODENTS
SOMATIC CELLS
VERTEBRATES
X RADIATION