Turnover of ribosomal RNA in mouse fibroblasts (3T3) in culture

Journal Article · Wed Jan 01 04:00:00 EST 1975 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(75)90146-9· OSTI ID:7263574

Kolodny, G M

Growing and confluent cultures of mouse fibroblasts were labeled with /sup 3/H-uridine and chased with an excess of nonradioactive uridine to investigate the turnover of ribosomal RNA. Growing cultures did not turn over their 18S and 28S ribosomal RNA; however, confluent cultures did show ribosomal RNA (rRNA) turnover. If the cells were labeled while growing and chased when confluent, 18S RNA displayed a two-component decay curve, while 28S RNA showed only single-component decay, similar in lifetime to the first component of the 18S RNA decay curve. If the cells were labeled while confluent, both the 18S and 28S RNA showed single-component decay curves with a lifetime possibly only slightly longer than the lifetime of the first component of the 18S RNA and the single component of the 28S RNA of the cultures labeled while growing.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 7263574
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 91; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CELL CULTURES
CONNECTIVE TISSUE CELLS
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
METABOLISM
MICE
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
RIBOSOMES
RNA
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM
URACILS
URIDINE
VERTEBRATES
YEARS LIVING RADIOISOTOPES