Reovirus-coded polypeptides in infected cells: isolation of two native monomeric polypeptides with affinity for single-stranded and double-stranded RNA, respectively

Journal Article · Thu Apr 01 04:00:00 EST 1976 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(76)90282-8· OSTI ID:7347589

Huismans, H; Joklik, W K

The cytoplasm of cells infected with rovirus contains 8 of the 10 polypeptides coded by this virus in free form in amounts equivalent to 1 to 10 percent of the progeny virus yield. All of these polypeptides exist in monomeric form except polypeptides ..mu..l and ..mu..2 which occur as dimers, the form in which they are present in virions. One of these polypeptides, sigma3, a constituent of the outer capsid shell, binds strongly to double-stranded RNA; it can be isolated in high yield in pure form by passing extracts of infected cells through columns of poly(IC)-cellulose and eluting. Another polypeptide, sigma2A, a nonstructural polypeptide, binds strongly to single-stranded RNA and appears to exist in infected cells complexed with RNA. It can be isolated in high yield in pure form by passing extracts of infected cells, suitably treated to dissociate these complexes, through columns of poly(A)-cellulose and eluting. The possible significance of the highly specific nucleic acid affinities of these two reovirus-coded polypeptides is discussed.

• Research Organization: Duke Univ., Durham, NC
• OSTI ID: 7347589
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 70:2; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BINDING ENERGY
CELL CONSTITUENTS
CHEMICAL ANALYSIS
CONNECTIVE TISSUE CELLS
CYTOPLASM
DNA
ENERGY
FIBROBLASTS
HELA CELLS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PEPTIDES
POLYPEPTIDES
PROTEINS
RNA
SOMATIC CELLS
VIRUSES