Distinct binding sites for zinc and double-stranded RNA in the reovirus outer capsid protein sigma3

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.1.273· OSTI ID:6925572

Schiff, L A; Nibert, M L; Co, M S; Brown, E G; Fields, B N

By atomic absorption analysis, the authors determined that the reovirus outer capsid protein sigma3, which binds double-stranded RNA (dsRNA), is a zinc metalloprotein. Using Northwestern blots and a novel zinc blotting technique, the authors localized the zinc- and dsRNA-binding activities of sigma3 to distinct V8 protease-generated fragments. Zinc-binding activity was contained within an amino-terminal fragment that contained a transcription factor IIIA-like zinc-binding sequence, and dsRNA-binding activity was associated with a carboxy-terminal fragment. By these techniques, new zinc- and dsRNA-binding activities were also detected in reovirus core proteins. A sequence similarity was observed between the catalytic site of the picornavirus proteases and the transcription factor IIIA-like zinc-binding site within sigma3. The authors suggest that the zinc- and dsRNA-binding activities of sigma3 may be important for its proposed regulatory effects on viral and host cell transcription and translation.

• Research Organization: Dept. of Microbiology and Molecular Genetics, Harvard Medical School (US)
• OSTI ID: 6925572
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:1; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201 -- Chemical & Physicochemical Properties
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
CHEMICAL ANALYSIS
CHEMICAL REACTION KINETICS
ELECTROPHORESIS
ELEMENTS
KINETICS
METALLOPROTEINS
METALS
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
REACTION KINETICS
RNA
TRANSCRIPTION FACTORS
VIRUSES
ZINC