Cell division requirement for activation of murine leukemia virus in cell culture by irradiation. [Gamma radiation]

Journal Article · Mon Mar 01 04:00:00 EST 1976 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(76)90237-3· OSTI ID:7347530

Otten, J A; Quarles, J M; Tennant, R W

Actively dividing cultures of AKR mouse cells were exposed to relatively low dose-rates of ..gamma.. radiation and tested for activation of endogenous leukemia viruses. Efficient and reproducible induction of virus was obtained with actively dividing cells, but cultures deprived of serum to inhibit cell division before and during ..gamma.. irradiation were not activated, even when medium with serum was added immediately after irradiation. These results show that cell division was required for virus induction but that a stable intermediate similar to the state induced by halogenated pyrimidines was not formed. In actively dividing AKR cell cultures, virus activation appeared to be proportional to the dose of ..gamma.. radiation; the estimated frequency of activation was 1-8 x 10/sup -/5 per exposed cell and the efficiency of activation was approximately 0.012 inductions per cell per rad. Other normal primary and established mouse cell cultures tested were not activated by ..gamma.. radiation. The requirement of cell division for radiation and chemical activation may reflect some common mechanism for initiation of virus expression.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 7347530
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 70:1; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL DIVISION
DISEASES
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
IRRADIATION
LEUKEMIA
LOW DOSE IRRADIATION
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
VIRUSES