Radioimmunoassay and chromatographic similarity of circulating endogenous gonadotropin releasing hormone and hypothalamic extracts in man. [/sup 125/I tracer technique]
- St. Bartholomew's Hospital, London
A highly sensitive radioimmunoassay for the gonadotropin releasing hormone has been developed in order to study its physiological importance in man. In view of the expected low concentrations in peripheral blood, large volumes of human plasma were extracted by two different methods and the characteristics of the radioimmunoassayable material compared with those of synthetic decapeptide and extracts of human hypothalami. The results indicate that radioimmunoassayable gonadotropin releasing hormone is present in some human plasmas but the plasma concentrations are less than 2.5 pg/ml. Peripheral levels were more consistently measurable in women at midcycle and after the menopause. The hormone was undetectable in the plasma of normal men, human cerebrospinal fluid, and fetal cerebral tissue, but was present in fetal hypothalami.
- OSTI ID:
- 7346947
- Journal Information:
- J. Clin. Endocrinol. Metab.; (United States), Vol. 43:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Production of specific antisera for radioimmunoassay of human luteinizing hormone (LH) in the presence of human chorionic gonadotropin (hCG). [/sup 125/I]
Luteinizing hormone-releasing hormone levels in human plasma: a radioimmunoassay method. [/sup 125/I]
Related Subjects
LH-RH
CHROMATOGRAPHY
RADIOIMMUNOASSAY
HYPOTHALAMUS
IODINE 125
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
550601* - Medicine- Unsealed Radionuclides in Diagnostics