Effect of hydrocortisone on cell morphology in C6 cells: the role of microfilaments in the inductive process. [Cytochalasin B; glycerol phosphate dehydrogenase]

Technical Report · Thu Jan 01 00:00:00 EST 1976

OSTI ID:7342752

Berliner, J A; Bennett, K; de Vellis, J

Hydrocortisone induced cell spreading and the formation of microfilaments in C6 cells with a time course similar to that found for the induction of GPDH. Cytochalasin B caused a rapid and reversible cell rounding and microfilament breakdown. Breakdown of both sheath and network microfilaments occurred within 1 hour; after removal of cytochalasin B, reversal of morphological effects began within 30 min. High calcium was found to block reversal of the cytochalasin B induced alteration, but did not affect shape or microfilaments in non-cytochalasin B treated cells. It is concluded that since microfilament induction occurs late and the block in synthesis of GPDH by cytochalasin B occurs within hours after addition, microfilaments induced by hydrocortisone are probably not important in the sequence of events leading to GPDH induction. But, however, microfilaments present in uninduced cells may be important in the sequence of GPDH induction, since treatment with cytochalasin B rapidly breaks these down and also inhibits GPDH induction by hydrocortisone.

• Research Organization: California Univ., Livermore (USA). Lawrence Livermore Lab.
• DOE Contract Number: E(04-1)-GEN-12
• OSTI ID: 7342752
• Report Number(s): UCLA-12-1095
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
MORPHOLOGICAL CHANGES
CALCIUM
BIOLOGICAL EFFECTS
DEHYDROGENASES
BIOSYNTHESIS
DRUGS
HYDROCORTISONE
ADRENAL HORMONES
ALKALINE EARTH METALS
CORTICOSTEROIDS
ELEMENTS
ENZYMES
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
METALS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PREGNANES
STEROID HORMONES
STEROIDS
SYNTHESIS
550300* - Cytology
550200 - Biochemistry