Effect of hydrocortisone on cell morphology in C6 cells
Journal Article
·
· J. Cell. Physiol.; (United States)
Hydrocortisone has been found to induce cell spreading in rat glial C6 cells by 24 hours after its addition. This spreading phenomenon is correlated with an increase in the fraction of the peripheral cytoplasm occupied by microfilaments. Cytochalasin B causes disorganization of microfilaments in the peripheral cytoplasm of the cells. Additionally, it also prevents cell spreading in response to hormonal stimulation. High levels of calcium prevent recovery of normal microfilament organization and cell spreading following removal of cytochalasin B, but have no effect on normal microfilament organization alone. Additionally both the hydrocortisone induced spreading of C6 cells and increases in peripheral microfilaments are shown to be dependent on RNA and protein synthesis. The levels of protein co-electrophorescing with actin are not affected by hydrocortisone.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 7208242
- Journal Information:
- J. Cell. Physiol.; (United States), Journal Name: J. Cell. Physiol.; (United States) Vol. 94:3; ISSN JCLLA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of hydrocortisone on cell morphology in C6 cells: the role of microfilaments in the inductive process. [Cytochalasin B; glycerol phosphate dehydrogenase]
Reversible inhibition of the hydrocortisone induction of glycerol phosphate dehydrogenase by cytochalasin B in rat glial C6 cells. [Model for mechanisms of action of steroids]
Special type of morphological reorganization induced by phorbol ester: reversible partition of cell into motile and stable domains
Technical Report
·
Wed Dec 31 23:00:00 EST 1975
·
OSTI ID:7342752
Reversible inhibition of the hydrocortisone induction of glycerol phosphate dehydrogenase by cytochalasin B in rat glial C6 cells. [Model for mechanisms of action of steroids]
Technical Report
·
Wed Dec 31 23:00:00 EST 1975
·
OSTI ID:7336247
Special type of morphological reorganization induced by phorbol ester: reversible partition of cell into motile and stable domains
Journal Article
·
Mon Jun 01 00:00:00 EDT 1987
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:5439718
Related Subjects
550000* -- Biomedical Sciences
Basic Studies
550800 -- Morphology
59 BASIC BIOLOGICAL SCIENCES
ACTIN
ADRENAL HORMONES
ALKALINE EARTH METALS
ANIMALS
BIOLOGICAL EFFECTS
CALCIUM
CELL CONSTITUENTS
CELL CULTURES
CORTICOSTEROIDS
CYTOPLASM
ELEMENTS
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
IN VITRO
KETONES
MAMMALS
METALS
MORPHOLOGICAL CHANGES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PREGNANES
PROTEINS
RATS
RNA
RODENTS
STEROID HORMONES
STEROIDS
SYNTHESIS
ULTRASTRUCTURAL CHANGES
VERTEBRATES
Basic Studies
550800 -- Morphology
59 BASIC BIOLOGICAL SCIENCES
ACTIN
ADRENAL HORMONES
ALKALINE EARTH METALS
ANIMALS
BIOLOGICAL EFFECTS
CALCIUM
CELL CONSTITUENTS
CELL CULTURES
CORTICOSTEROIDS
CYTOPLASM
ELEMENTS
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
IN VITRO
KETONES
MAMMALS
METALS
MORPHOLOGICAL CHANGES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PREGNANES
PROTEINS
RATS
RNA
RODENTS
STEROID HORMONES
STEROIDS
SYNTHESIS
ULTRASTRUCTURAL CHANGES
VERTEBRATES