Simultaneous radioimmunoassay of secretin and gastrin. [/sup 125/I and /sup 131/I tracer techniques]
Journal Article
·
· Anal. Biochem.; (United States)
Radioimmunoassay techniques for the measurement of gastrointestinal hormones have been modified to allow simultaneous assay of both secretin and gastrin in the same plasma samples. The system employs antibodies with high specificity for these two hormones and both /sup 125/I-labeled secretin and /sup 131/I-labeled gastrin as markers. Separation of the free from the bound form of the hormones was achieved by plasma- and dextran-coated charcoal. The unique separation method has been found to be independent of the uniformity of plasma samples. The sensitivity and the reliability of the simultaneous assays were comparable to those of the single assays. The fasting levels of human plasma secretin and gastrin obtained by the simultaneous assay were 62.9 +- 3.6 and 43.5 +- 2.8 pg/ml, respectively.
- Research Organization:
- Genesee Hospital, Rochester, NY
- OSTI ID:
- 7341207
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 74:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GASTRIN
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SECRETIN
TRACER TECHNIQUES
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GASTRIN
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SECRETIN
TRACER TECHNIQUES