Factors involved in the regulation of early enzyme synthesis and lysis of Escherichia coli B infected with bacteriophage T4D

Technical Report · Thu Jan 01 00:00:00 EST 1976

OSTI ID:7339204

Thompson, S

The capability of T4/sup +/-infected Escherichia coli cells to display handling-induced lysis may implicate the activity of a known, untested gene, or a heretofore undiscovered gene. This gene product may be involved in the normal lytic process; it may be a new phage-induced phospholipase or a late function that activates a latent E. coli phospholipase. Another possibility is that the late function is involved in membrane biosynthesis or modification. Alternatively, the required late protein(s) may have some other role that only secondarily affects the cell's permeability barrier. Whatever the case may be, this work is of practical interest since chilling and centrifugation of infected cells are very common procedures and researchers should be aware of any handling-induced damage.

• Research Organization: Rochester Univ., N.Y. (USA). School of Medicine and Dentistry
• DOE Contract Number: E(11-1)-3055
• OSTI ID: 7339204
• Report Number(s): UR-3055-16
• Resource Relation: Other Information: Thesis
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIOPHAGES
BIOLOGICAL EFFECTS
ESCHERICHIA COLI
ENZYMES
PROTEINS
BIOSYNTHESIS
BIOCHEMICAL REACTION KINETICS
GENETIC CONTROL
METABOLISM
BACTERIA
KINETICS
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PEST CONTROL
REACTION KINETICS
SYNTHESIS
VIRUSES
550700* - Microbiology
550200 - Biochemistry