Late effect of bacteriophage T4D on the permeability barrier of Escherichia coli

Journal Article · Wed Feb 01 04:00:00 EST 1978 · J. Virol.; (United States)

OSTI ID:7088038

Thompson, S; Wiberg, J S

Cold centrifugation of lysis-inhibited Escherichia coli B infected with wild-type T4D results in extensive lysis beginning around 20 min after infection at 37/sup 0/C. Infection with an e mutant, which fails to make lysozyme, prevents lysis, but does not prevent a marked loss of K/sup +/ and Mg/sup 3 +/. The t gene product, thought to disrupt the cytoplasmic membrane in natural lysis, is not required for this handling-induced cation loss or lysis. Three lines of evidence argue that late protein synthesis is required to develop this potential for cation loss; the potential does not develop in infections by: (1) mutants defective in DNA synthesis, (2) mutants defective in gene 55, and (3) wild-type T4 when chloramphenicol is added at 6 min after infection. All late mutants examined, which are blocked in the major pathways of morphogenesis, do not prevent development of the potential. The evidence argues for a new, late effect of T4 infection on the cytoplasmic membrane.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 7088038
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 25:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALKALI METALS
ALKALINE EARTH METALS
ANTIBIOTICS
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHLORAMPHENICOL
DNA
DRUGS
ELEMENTS
ENZYMES
ESCHERICHIA COLI
GENES
GLYCOSYL HYDROLASES
HYDROLASES
IONS
LYSIS
LYSOZYME
MAGNESIUM
MEMBRANES
METALS
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PERMEABILITY
POTASSIUM
PROTEINS
SYNTHESIS
VIRUSES