Properties of uvrE mutants of Escherichia coli K12. Part 1. Effects of uv irradiation on DNA metabolism
Escherichia coli K12 uvrE is a mutator strain which is highly sensitive to ultraviolet radiation. In an attempt to determine the underlying molecular basis for the UV sensitivity, a mutant and an isogenic wild type strain were compared with regard to several metabolic responses to 254 nm radiation. The introduction of single strand breaks into intracellular DNA after irradiation is normal; however, the rate of excision of pyrimidine dimers as well as of DNA degradation and final rejoining of the strand breaks is lower in the mutant as compared to the repair proficient strain. These data suggest that the uvrE gene product may be involved in a reaction between the incision and excision steps in the excision repair process. (Author) (GRA)
- Research Organization:
- Rijksverdedigingsorganisatie TNO, Rijswijk (Netherlands). Medisch Biologisch Lab. TNO
- OSTI ID:
- 7339182
- Report Number(s):
- N-75-24332; MBL-1974-25; TDCK-65293
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
MICROORGANISMS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION