Chemical radiosensitization and quality of cellular damage in bacteria exposed to gamma rays. [Streptococcus faecalis]

Journal Article · Sun Aug 01 04:00:00 EDT 1976 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574426· OSTI ID:7339168

Nair, C K.K.; Pradhan, D S; Sreenivasan, A

Iodoacetic acid (IAA) and N-ethylmaleimide (NEM) when present during exposure of Streptococcus faecalis cells to gamma radiation enhance radiation-induced lethality under both anoxic and aerated conditions. The changes brought about by this radiosensitization in cellular functions have been studied with a view to elucidating the mechanism responsible for the increased loss of viability. The quality of cellular damage in chemical radiosensitization was investigated by correlating survival and the biosynthetic capacity of an irradiated cell population. The relationship between surviving fraction and extent of incorporation of /sup 3/H-thymidine into DNA was found to be unaffected regardless of whether the sensitizers (IAA or NEM) were present or absent during irradiation under anoxia. However, under the oxic condition of irradiation the survival--DNA-labeling relationship was completely different in the presence and in the absence of the sensitizers.

• Research Organization: Bhabha Atomic Research Centre, Bombay
• OSTI ID: 7339168
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 67:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETIC ACID
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACIDS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
IMIDES
IONIZING RADIATIONS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NEM
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RADIOSENSITIZERS
RESPONSE MODIFYING FACTORS
STREPTOCOCCUS
SURVIVAL TIME