Bacteriophage PBS2-induced inhibition of uracil-containing DNA degradation. [Bacillus subtilis]

Journal Article · Mon Nov 01 04:00:00 EST 1976 · J. Virol.; (United States)

OSTI ID:7338303

Katz, G E; Price, A R; Pomerantz, M J

Degradation of uracil-containing DNA by Bacillus subtilis extracts and its inhibition after infection by the uracil-containing DNA phage PBS2 have been investigated to resolve differences between the published reports of Tomita and Takahashi (1975) and Friedberg, et al. (1975, 1976). The product of hydrolysis of PBS2 DNA, tritium labeled in its uracil and cytosine residues, is solely uracil and not deoxyuridine. The degrading activity is completely inhibited within 7 min after PBS2 infection, before any other known PBS2-induced protein is detectable. The production of the PBS2 inhibitor (a small, heat-stable protein) continues until 10 to 20 min postinfection.

• Research Organization: Univ. of Michigan, Ann Arbor
• OSTI ID: 7338303
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 20:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
AZINES
BACILLUS
BACILLUS SUBTILIS
BACTERIA
BACTERIOPHAGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
CYTOSINE
DECOMPOSITION
DNA
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROLYSIS
HYDROXY COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LYSIS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PARASITES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
REACTION KINETICS
SOLVOLYSIS
TRACER TECHNIQUES
TRITIUM
URACILS
VIRUSES
YEARS LIVING RADIOISOTOPES