Synthesis of DNA containing uracil during bacteriophage infection of Bacillus subtilis. Technical progress report, August 1, 1975--July 31, 1976

Technical Report · Thu Apr 01 04:00:00 EST 1976

DOI:https://doi.org/10.2172/7234162· OSTI ID:7234162

Price, A. R.

We have studied the synthesis of proteins and nucleic acids to understand how the bacteriophage PBS2 can make uracil-containing DNA in a cell which normally makes thymine-containing Bacillus subtilis DNA. We showed that the earliest known PBS2-induction of a protein is the inhibitor of the B. subtilis N-glycosidase (or uracil-DNA nuclease). This enzyme cleaves uracil from PBS2 DNA, and it is inhibited before any other known PBS2 protein appears in infected cells. The PBS2 dUMP kinase, which probably provides dUTP for uracil-DNA synthesis, has been partially characterized. Purification and induction in polymerase I- and III-deficient host cells indicate that the PBS2 DNA polymerase is a new enzyme. However, infection of temperature-sensitive polymerase III mutants suggests that the host's DNA polymerase III may play some role in PBS2 replication, at least under certain conditions. Two-dimensional thin-layer chromatography methods have been developed to quantitate the deoxyribonucleoside triphosphate pools before and after PBS2 infection.

• Research Organization: Michigan Univ., Ann Arbor (USA). Dept. of Biological Chemistry
• Sponsoring Organization: US Energy Research and Development Administration (ERDA)
• OSTI ID: 7234162
• Report Number(s): COO-2101-27
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AZINES
BACILLUS
BACILLUS SUBTILIS
BACTERIA
BACTERIOPHAGES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
DNA
ENZYMES
HETEROCYCLIC COMPOUNDS
HOST-CELL REACTIVATION
HYDROLASES
HYDROXY COMPOUNDS
INFECTIVITY
KINETICS
METABOLISM
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
REACTION KINETICS
RECOVERY
REPAIR
SYNTHESIS
URACILS
VIRUSES