Changes in membrane receptors of B and T human lymphocytes exposed to /sup 60/Co gamma rays

Journal Article · Mon Nov 01 04:00:00 EST 1976 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574485· OSTI ID:7338252

Facchini, A ^[1]; Maraldi, N M; Bartoli, S; Farulla, A; Manzoli, F A

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1. Univ., Bologna

The present report describes the effect of 50 to 2500 rad of /sup 60/Co gamma rays on the membrane receptors of B and T lymphocytes. Surface membrane immunoglobulins and Fc receptors were used as markers for B cells, while the formation of rosettes with sheep red blood cells was used as a marker for T cells. The effect of radiation is expressed as the percentage of viable cells that bear membrane receptors. The radiation effects were studied after 2, 24, 36 hr of culture. For B lymphocytes no reduction in SmIg and Fc receptors was seen 2 hr after radiation, whereas a dose-related reduction was observed after 24 and 36 hr. A dose-related inhibition of the ''capping'' of SmIg was observed. For T lymphocytes a reduction of E rosette formation was seen starting 2 hr after irradiation. Electron microscopy of separated B and T cells showed a higher sensitivity of T cells as well as a different structural arrangement of the chromatin as a consequence of radiation damage. These data suggest differentiated behavior of B and T lymphocyte membrane receptors after exposure to radiation.

• OSTI ID: 7338252
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 68:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATIN
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ELECTRON MICROSCOPY
GAMMA RADIATION
GLOBULINS
IMMUNOGLOBULINS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MEMBRANES
MICROSCOPY
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
SOMATIC CELLS