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Temperature-sensitive mutant of Schizosaccharomyces pombe exhibiting enhanced radiation sensitivity. [Gamma and UV radiation]

Journal Article · · J. Bacteriol.; (United States)
OSTI ID:7338242
; ;
A conditional lethal and radiation-sensitive mutant of Schizosaccharomyces pombe is described in which both characteristics result from a single gene mutation. Confirmation of the pleiotropic nature of this mutation was obtained by tetrad analysis and by testing the radiation sensitivity of a large number of revertants that grew normally at the restrictive temperature. The colony-forming ability of the mutant after ultraviolet radiation, ..gamma.. radiation, and ethyl methane sulfonate treatment is considerably altered by the post-treatment incubation temperature, showing higher survival at 25 than at 30/sup 0/C. The radiosensitivity of the mutant is also influenced by the stage of growth. The difference in radiation sensitivity between the wild type and mutant is greater when log-phase cultures are compared. The characteristics of this mutant suggest that it is defective in a step common to both deoxyribonucleic acid replication and repair.
Research Organization:
National Research Council of Canada, Ottawa
OSTI ID:
7338242
Journal Information:
J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 128:2; ISSN JOBAA
Country of Publication:
United States
Language:
English

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Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOMASS
CFU
DNA
ELECTROMAGNETIC RADIATION
EMS
ENERGY SOURCES
ESTERS
FUNGI
GAMMA RADIATION
IONIZING RADIATIONS
MICROORGANISMS
MUTAGENS
MUTANTS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RENEWABLE ENERGY SOURCES
SULFONIC ACID ESTERS
SURVIVAL TIME
TEMPERATURE EFFECTS
ULTRAVIOLET RADIATION
YEASTS