Mating-type mutations in Schizosaccharomyces pombe: isolation of mutants and analysis of strains with an h/sup -/ or h/sup +/ phenotype. [UV radiation]

Journal Article · Tue Jun 01 04:00:00 EDT 1976 · Genetics; (United States)

OSTI ID:7362342

Meade, J H; Gutz, H

Mutants defective in various steps of the sexual cycle have been isolated from homothallic strains of Schizosaccharomyces pombe. These mutants include heterothallic h+ and h- strains. We have isolated additional h+ and h- mutants from homothallic strains. Those mutants which are due to mutations in the mating-type region were analyzed in detail. Our resutls show that the mating-type gene mat2 not only has a function in copulation and meiosis, but that it also regulates the formation of the map1 gene product (map1 is a mating-type auxiliary gene). Some of the h- mutants have lost only one of the three functions while others are defective in at least two, perhaps all three, functions. Further, we show that the mat1- allele of h/sup 90/ strains can mutate to mat1+ but that mutations in mat2 appear to affect the mutational behavior of mat1. Finally, we describe a new inactive mating-type allele mat2*, which is different from mat2/sup 0/ in that it can mutate to mat2+.

• Research Organization: Univ. of Texas, Dallas
• OSTI ID: 7362342
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 83:2; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOMASS
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
FUNGI
MICROORGANISMS
MUTANTS
MUTATIONS
PHENOTYPE
PLANTS
RADIATION EFFECTS
RADIATIONS
RENEWABLE ENERGY SOURCES
ULTRAVIOLET RADIATION
YEASTS