Effects of tritium-labeled pyrimidine nucleosides on epithelial cell proliferation in the mouse. I. Cytodynamic perturbations in normal circadian rhythms after a single injection of /sup 3/H-thymidine

Journal Article · Mon Nov 01 04:00:00 EST 1976 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574477· OSTI ID:7338159

Olsson, L

In a combined stathmokinetic, autoradiographic, and microspectrophotometric study on the cytodynamics in mouse epidermis, cirdardian rhythms were observed in the number of cells within the various phases (except G/sub 2/) of the mitotic cell cycle, and in mitotic rate. S-Phase influx and efflux were computed and were found to vary during a 24-hr period. No diurnal rhythm was observed in mitotic rate in the epithelium of small intestine. /sup 3/H-Thymidine (1 ..mu..Ci/g; sp act 5 Ci/mmol) induced mitotic delay in labeled cells 2 to 8 hr after administration. A supranormal mitotic activity in unlabeled cells was observed 12 to 24 hr after injection of /sup 3/H-TdR. ''Cold'' thymidine had no effects on epidermal cell proliferation. S-Phase duration and average cell cycle time were estimated by traditional cell kinetic methods. The results suggest a /sup 3/H-TdR-induced synchrony in cells entering the S phase 0 to 15 hr after injection of the precursor. A mitotic delay was seen in small intestinal epithelium 2 to 6 hr after injection of the precursor.

• Research Organization: Univ. of Copenhagen
• OSTI ID: 7338159
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 68:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560162* -- Radionuclide Effects
Kinetics
& Toxicology-- Animals
Plants
Microorganisms
& Cells
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CYCLE
CELL DIVISION
CELL PROLIFERATION
DAILY VARIATIONS
DIGESTIVE SYSTEM
EPIDERMIS
EPITHELIUM
GASTROINTESTINAL TRACT
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
INTESTINES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICE
MITOSIS
MITOTIC DELAY
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION EFFECTS
RADIOISOTOPES
RIBOSIDES
RODENTS
SKIN
SMALL INTESTINE
THYMIDINE
TISSUES
TRITIUM
VARIATIONS
VERTEBRATES
YEARS LIVING RADIOISOTOPES