Effects of tritium-labeled pyrimidine nucleosides on epithelial cell proliferation in the mouse. I. Cytodynamic perturbations in normal circadian rhythms after a single injection of /sup 3/H-thymidine
In a combined stathmokinetic, autoradiographic, and microspectrophotometric study on the cytodynamics in mouse epidermis, cirdardian rhythms were observed in the number of cells within the various phases (except G/sub 2/) of the mitotic cell cycle, and in mitotic rate. S-Phase influx and efflux were computed and were found to vary during a 24-hr period. No diurnal rhythm was observed in mitotic rate in the epithelium of small intestine. /sup 3/H-Thymidine (1 ..mu..Ci/g; sp act 5 Ci/mmol) induced mitotic delay in labeled cells 2 to 8 hr after administration. A supranormal mitotic activity in unlabeled cells was observed 12 to 24 hr after injection of /sup 3/H-TdR. ''Cold'' thymidine had no effects on epidermal cell proliferation. S-Phase duration and average cell cycle time were estimated by traditional cell kinetic methods. The results suggest a /sup 3/H-TdR-induced synchrony in cells entering the S phase 0 to 15 hr after injection of the precursor. A mitotic delay was seen in small intestinal epithelium 2 to 6 hr after injection of the precursor.
- Research Organization:
- Univ. of Copenhagen
- OSTI ID:
- 7338159
- Journal Information:
- Radiat. Res.; (United States), Vol. 68:2
- Country of Publication:
- United States
- Language:
- English
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ANIMAL CELLS
MITOTIC DELAY
CELL CYCLE
DAILY VARIATIONS
CELL PROLIFERATION
EPIDERMIS
NUCLEOSIDES
BIOLOGICAL EFFECTS
PYRIMIDINES
BIOLOGICAL RADIATION EFFECTS
MICE
MITOSIS
SMALL INTESTINE
THYMIDINE
TRITIUM
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL DIVISION
DIGESTIVE SYSTEM
EPITHELIUM
GASTROINTESTINAL TRACT
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
INTESTINES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIOISOTOPES
RIBOSIDES
RODENTS
SKIN
TISSUES
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VERTEBRATES
YEARS LIVING RADIOISOTOPES
560162* - Radionuclide Effects
Kinetics
& Toxicology- Animals
Plants
Microorganisms
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