Genetic analysis of aspartate aminotransferase isozymes from hybrids between Drosophila melanogaster and Drosophila simulans and mutagen-induced isozyme variants

Journal Article · Sun Aug 01 04:00:00 EDT 1976 · Genetics; (United States)

OSTI ID:7337496

Grell, E H

The aspartate aminotransferases (designated GOT1 and GOT2) are two enzymes of Drosophila melanogaster for which naturally occurring electrophoretic variants were not found. There is an electrophoretic difference between D. melanogaster and D. simulans. Since the F/sub 1/ hybrid offspring of these species are sterile, a genetic analysis of the ordinary type cannot be done on differences between the two species. A method was devised to make ''partial hybrids'' in which one chromosome arm is homozygous for melanogaster genes in an otherwise hybrid background. By using this method, Got1 was localized to 2R and Got2 to 2L. Once a gene can be assigned to a chromosome, it may be followed in crossing schemes and mutations from mutagen treatments may be expected. At the locus of Got1 a mutation with low activity was recovered and designated Got1/sup lo/. It was located at a genetic map position of 75 on 2R. A Got2 mutant with a greater migration to the anode was recovered and designated Got2/sup J/. It was located at a genetic map position of 3.0, and in the salivary chromosome was between 22B1 and 22B4 inclusive.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 7337496
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 83:4; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINOTRANSFERASES
ANIMALS
ARTHROPODS
BIOLOGY
CHROMOSOMES
DROSOPHILA
ENZYMES
FLIES
FRUIT FLIES
GENETICS
HYBRIDIZATION
INSECTS
INVERTEBRATES
ISOENZYMES
MUTATIONS
TRANSFERASES