Inactivation of catalase by near ultraviolet light and tryptophan photoproducts
Journal Article
·
· Mol. Cell. Biochem.; (United States)
Certain ocular proteins have been found to be chemically modified by exposure to near-uv light (320 to 390 nm) in the presence of tryptophan. Colored and fluorescent tryptophan photoproducts bind firmly to proteins, thereby altering their physico-chemical properties. The question of whether such a reaction would inhibit the catalytic action of catalase is herein raised. When solutions of bovine liver catalase were re-incubated up to 24 hr under near-uv with preirradiated tryptophan and dialyzed, most of the ability of the enzyme to decompose H/sub 2/O/sub 2/ was lost. Similar results occurred for catalase activities of bovine cornea and lens epithelia. The enzyme protein exhibited altered uv absorption and fluorescence spectra and increased electrophoretic mobility after binding photoproducts. Near-uv light photoproducts of tryptophan are thus capable of deactivating crystalline and tissue catalase.
- Research Organization:
- Univ. of Rochester, NY
- OSTI ID:
- 7334010
- Journal Information:
- Mol. Cell. Biochem.; (United States), Journal Name: Mol. Cell. Biochem.; (United States) Vol. 11:3; ISSN MCBIB
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:4344447
Related Subjects
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOXYLIC ACIDS
CATALASE
CHEMISTRY
ELECTROMAGNETIC RADIATION
ENZYMES
EYES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INACTIVATION
INDOLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHOTOCHEMISTRY
PROTEINS
PYRROLES
RADIATION EFFECTS
RADIATIONS
SENSE ORGANS
SPECTRA
TRYPTOPHAN
ULTRAVIOLET RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOXYLIC ACIDS
CATALASE
CHEMISTRY
ELECTROMAGNETIC RADIATION
ENZYMES
EYES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INACTIVATION
INDOLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHOTOCHEMISTRY
PROTEINS
PYRROLES
RADIATION EFFECTS
RADIATIONS
SENSE ORGANS
SPECTRA
TRYPTOPHAN
ULTRAVIOLET RADIATION