Analysis of parameters affecting the solid phase radioimmunoassay quantitation of antibody to meningococcal antigens. [/sup 125/I tracer technique]

Journal Article · Mon Nov 29 23:00:00 EST 1976 · J. Immunol.; (United States)

OSTI ID:7326428

Zollinger, W D; Dalrymple, J M; Artenstein, M S

An indirect solid phase radioimmunoassay (SPRIA) performed in flexible polyvinyl microtiter plates was modified for quantitative determination of antibodies to various bacterial antigens. Parameters affecting quantitation of the assay were investigated by using meningococcal serotype antigens and rabbit antisera to whole meningococci as a model system. Binding of purified antigens to the plate was essentially complete after 1 hr at 37/sup 0/C, and only 4 to 8 percent of the bound antigen was released during the course of the assay. About 90 percent of the specific primary antibody (PAb) added to the antigen-coated wells was bound after overnight incubation at room temperature, whereas, 10 to 25 percent of the bound PAb was released before completion of the assay. Under conditions of limiting PAb the time required for saturation binding of /sup 125/I-anti-immunoglobulin (secondary antibody = SAb) to the PAb was dependent on the concentration of SAb.

• Research Organization: Walter Reed Army Inst. of Research, Washington, DC
• OSTI ID: 7326428
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 117:5; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL MODELS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MENINGOCOCCUS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES