Microtiter solid-phase radioimmunoassay for hepatitis B antigen

Journal Article · Mon Oct 01 04:00:00 EDT 1973 · Appl. Microbiol., v. 26, no. 4, pp. 478-484

OSTI ID:4335493

Purcell, R H; Wong, D C; Alter, H J; Holland, P V

A micro-solid-phase radioimmunoassay (micro-SPRIA) for hepatitis B antigen (HB Ag) was developed for use with microtiter serological equipment. Radiolabeled immunoglobulin G was prepared from human and animal sera containing hepatitis B antibody (HB Ab); it was not necessary to isolate specffic HB Ab by immunochemical means. A micro-SPRIA prepared with guinea pig reagents was approximately as sensitive as the AusRIA radioimmunoassay, but, like the AusRIA test, yielded false positive results. A micro-SPRIA prepared with human reagents was slightly less sensitive but did not yield false positive results. These micro-SPRIA tests offer several advantages, including conservation of reagents, adaptability to other antigen-antibody systems, ease of performance (especially when testing large numbers of specimens), and economy. (auth)

• Research Organization: Lab. of Infectious Diseases, Bethesda, Md
• NSA Number: NSA-29-024573
• OSTI ID: 4335493
• Journal Information: Appl. Microbiol., v. 26, no. 4, pp. 478-484, Journal Name: Appl. Microbiol., v. 26, no. 4, pp. 478-484; ISSN APMBA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*ANTIGENS-- RADIOIMMUNOASSAY
ANTIBODIES
BLOOD SERUM
HEPATITIS
IMMUNOGLOBULINS
IODINE 125
N48830* --Life Sciences--New Tracer Techniques--Other
TRACER TECHNIQUES