Microtiter solid-phase radioimmunoassay for hepatitis A antigen and antibody

Journal Article · Sun Feb 01 04:00:00 EST 1976 · J. Immunol., v. 116, no. 2, pp. 349-356

OSTI ID:4042523

Purcell, R H; Wong, D C; Moritsugu, Y; Dienstag, J L; Routenberg, J A; Boggs, J D

A microtiter solid phase radioimmunoassay for hepatitis A antigen (HA Ag) and antibody (anti-HA) was developed. The test was more sensitive than immune adherence hemagglutination for detecting HA Ag and almost as sensitive for detecting anti-HA. The specificity and sensitivity of reagents were examined and optimum conditions for the test were determined. Radioimmunoassay, immune adherence hemagglutination, and immune electron microscopy were compared for detecting anti-HA. A serologic response to HA Ag was detected in paired sera from patients with type A hepatitis but not from patients with type B or non-A, non-B hepatitis by all three techniques. (auth)

• Research Organization: National Inst. of Allergy and Infectious Diseases, San Diego, CA
• NSA Number: NSA-33-029949
• OSTI ID: 4042523
• Journal Information: J. Immunol., v. 116, no. 2, pp. 349-356, Journal Name: J. Immunol., v. 116, no. 2, pp. 349-356; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

*ANTIBODIES-- RADIOIMMUNOASSAY
*ANTIGENS-- RADIOIMMUNOASSAY
550201* --Biomedical Sciences
Basic Studies--Biochemistry-- Tracer Techniques
ELECTRON MICROSCOPY
HEPATITIS
IMMUNE SERUMS
IODINE 125
N48830* --Life Sciences--New Tracer Techniques--Other
TRACER TECHNIQUES