Radioimmunoassay of lysergic acid diethylamide (LSD) in serum and urine by using antisera of different specificities

Journal Article · Tue Feb 01 04:00:00 EST 1977 · Clin. Chem.; (United States)

OSTI ID:7325925

Ratcliffe, W A ^[1]; Fletcher, S M; Moffat, A C; Ratcliffe, J G; Harland, W A; Levitt, T E

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1. Stobhill General Hospital, Glasgow

We raised high-titre antisera to two LSD-bovine serum albumin conjugates, one linked via the indole nitrogen, the other via the amide side-chain. The antisera were specific for different parts of the LSD molecule, as demonstrated by cross-reactivity studies with LSD, its metabolites, ergot alkaloids, and closely related compounds. The antisera were used to develop a double-antibody radioimmunoassay with a detection limit of about 0.4 ..mu..g of LSD per liter of unextracted urine or serum. We saw no nonspecific interference by urine, serum, or from a series of commonly used drugs. There was good correlation between immunoassay values obtained with the two antisera (r = 0.91). However, the antiserum linked via the indole nitrogen gave consistently higher results for samples from persons who had taken LSD, owing to greater cross-reactivity with LSD metabolites. Radioimmunoassay by use of two such antisera is a more specific screening procedure for LSD abuse than has been available previously. In addition, antisera cross-reacting with LSD metabolites allow measurement of these compounds, for which there is no satisfactory method at the concentrations found in biological fluids in man.

• OSTI ID: 7325925
• Journal Information: Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 23:2; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD SERUM
BODY FLUIDS
DRUGS
HALLUCINOGENS
IMMUNE SERUMS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MAN
PRIMATES
PSYCHOTROPIC DRUGS
RADIOASSAY
RADIOIMMUNOASSAY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINE
VERTEBRATES
WASTES