Characterization of postreplication repair in mutagen-sensitive strains of Drosophila melanogaster. [X radiation]

Journal Article · Mon Nov 01 04:00:00 EST 1976 · Genetics; (United States)

OSTI ID:7323918

Boyd, J B; Setlow, R B

Mutants of Drosophila melanogaster, with suspected repair deficiencies, were analyzed for their capacity to repair damage induced by x-rays, and uv radiation. Analysis was performed on cell cultures derived from embryos of homozygous mutant stocks. Postreplication repair following uv radiation has been analyzed in mutant stocks derived from a total of ten complementation groups. Cultures were irradiated, pulse-labeled, and incubated in the dark prior to analysis by alkaline sucrose gradient centrifugation. Kinetics of the molecular weight increase in newly synthesized DNA were assayed after cells had been incubated in the presence or absence of caffeine. Two separate pathways of postreplication repair have been tentatively identified by mutants derived from four complementation groups. The proposed caffeine sensitive pathway (CAS) is defined by mutants which also disrupt meiosis. The second pathway (CIS) is caffeine insensitive and is not yet associated with meiotic functions. All mutants deficient in postreplication repair are also sensitive to nitrogen mustard. The mutants investigated display a normal capacity to repair single-strand breaks induced in DNA by x-rays, although two may possess a reduced capacity to repair damage caused by localized incorporation of high specific activity thymidine-/sup 3/H. The data have been employed to construct a model for repair of uv-induced damage in Drosophila DNA. Implications of the model for DNA repair in mammals are discussed.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 7323918
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 84:3; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560153* -- Radiation Effects on Animals-- Invertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMINES
ANIMAL CELLS
ANIMALS
ANTIMITOTIC DRUGS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CAFFEINE
CELL CULTURES
CELL DIVISION
DNA
DROSOPHILA
DRUGS
ELECTROMAGNETIC RADIATION
EMBRYONIC CELLS
FLIES
FRUIT FLIES
HETEROCYCLIC COMPOUNDS
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
MEIOSIS
MOLECULAR WEIGHT
MUTANTS
NITROGEN MUSTARD
NUCLEIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
STRAND BREAKS
SYNTHESIS
ULTRAVIOLET RADIATION
X RADIATION
XANTHINES