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Cytokinetics of regeneration in. beta. -irradiated guines pig epidermis

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3574716· OSTI ID:7323799
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We studied the time course of depopulation and repopulation of epidermal cells in guinea-pig skin after ..beta.. irradiation from a /sup 90/Sr-/sup 90/Y scaled source. Immediately after clipping, one site on each flank was locally irradiated with 1000, 2200, 3000, or 5000 rad (six sites per animal). The changes in number of basal cells, labeling index (LI), and the appearance of clusters of /sup 3/H-thymidine-labeled basal cells (two or more pulse-labeled cells in direct contact) were observed. Maximum cell loss of recognizable basal cells of 20 percent on Day 8, 60 percent on Day 12, and 75 percent on Day 15 occurred after irradiation with 1000, 2200, and 3000 rad, respectively. The data for 5000 rad was similar to that in 3000-rad-irradiated epidermis. Repopulation (as evidenced by increase in LI and clusters of labeled cells) at all doses began on about the day of maximal cell loss. Regeneration occurred from survivors within the irradiated area after 1000 and 2200 rad, and was completed in 5 days (i.e., on Days 13 and 17, respectively). Reepithelization from the radiation margins occurred after 3000 and 5000 rad ..beta.. irradiation beginning on about Day 15 and was completed in about 7 days. Labeled cells in clusters were rarely seen in nonirradiated epidermis. In irradiated tissue, however, clusters paralleled the rise in LI and reached peaks at about the time of maximal cell loss and then returned to control level at Days 23 and 27 after 1000 and 2200 rad. They remained somewhat above the control level to the 33rd day after 3000 rad (three clusters/mm). There was no hyperplasia after 1000 rad, but a long-lived hyperplastic epidermis resulted after higher doses.

Research Organization:
Albert Einstein Medical Center, Philadelphia
OSTI ID:
7323799
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 71:1; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA PARTICLES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BODY
CHARGED PARTICLES
DOSE-RESPONSE RELATIONSHIPS
EPIDERMIS
EPITHELIUM
GUINEA PIGS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION EFFECTS
RADIOISOTOPES
RECOVERY
RIBOSIDES
RODENTS
SKIN
THYMIDINE
TISSUES
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES