Movement of beta-irradiated epidermal basal cells to the spinous-granular layers in the absence of cell division

Conference · Wed Jan 01 04:00:00 EST 1975 · J. Invest. Dermatol., v. 64, no. 6, pp. 431-435

OSTI ID:4136711

Etoh, H; Taguchi, Y H; Tabachnick, J

Guinea-pig epidermis was irradiated with 3000 rad of beta rays 1 hr after two injections of [$sup 3$H]thymidine 5 hr apart (labeled cells in S phase and G$sub 2$ phase) or 18 hr after injection (labeled early G$sub 1$ cells). In nonirradiated epidermis labeled basal cells divided within 24 hr with daughter cells remaining in the basal layer, and approximately 50 percent of the labeled cells moved into the spinal layer by the 3rd day. Cell division in nonirradiated epidermis diluted the number of silver grains/nucleus, and lightly labeled cells were found in the granular layer by day 7. Beta irradiation inhibited cell division but it did not slow the rate of transit (ca 8 days) of irradiated labeled cells from basal to granular layer, some of these remaining heavily labeled. Although cell division may play some role in upward movement of basal cells in normal epidermis detachment of a basal cell from the basement membrane and its transit to the granular layer is unimpaired in the absence of cell division. These findings suggest that some radioresistant metabolic function(s), not cell division, is responsible for upward movement of basal cells. (auth)

• Research Organization: Albert Einstein Medical Center, Philadelphia
• NSA Number: NSA-33-003249
• OSTI ID: 4136711
• Conference Information: Journal Name: J. Invest. Dermatol., v. 64, no. 6, pp. 431-435
• Country of Publication: United States
• Language: English

Related Subjects

*ANIMAL CELLS-- BIOLOGICAL RADIATION EFFECTS
*CELL DIVISION-- BIOLOGICAL RADIATION EFFECTS
560152* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Vertebrates
BETA PARTICLES
EPIDERMIS
GUINEA PIGS
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates