Application of the blind assay to biological activity and tobacco smoke terpenes

Journal Article · Wed Dec 01 04:00:00 EST 1976 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac50008a044· OSTI ID:7319659

Ho, C H; Griest, W H; Guerin, M R

The blind assay technique was applied to the chromatographic data generated from gas-liquid chromatography (GLC) profiles of terpene-enriched fractions from the total particulate matter (TPM) of eight experimental cigarettes. Correlation of the entire terpene fraction peak area and 16 individual peaks with data from three skin-painting bioassay parameters indicated that the terpene fraction as a whole and at least 12 of its constituents correlate well with biological activity. Six correlating peaks were tentatively identified as d-limonene, damascenone, norphytene, neophytadiene, phytol, and squalene. High correlations were also observed between the TPM concentrations of these constituents and the total terpene fraction. Of the terpenes visualized, d-limonene would act as the best indicator of the tobacco smoke biological activity.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 7319659
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 48:14; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
BIOLOGICAL EFFECTS
CHROMATOGRAPHY
COLLOIDS
DATA ANALYSIS
DISPERSIONS
ORGANIC COMPOUNDS
PARTICLES
RESIDUES
SEPARATION PROCESSES
SMOKES
SOLS
TERPENES
TOBACCO SMOKES