Assessment of the carcinogenic N-nitrosodiethanolamine in tobacco products and tobacco smoke

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/2.11.1123· OSTI ID:5310902

Brunnemann, K D; Hoffmann, D

A simple, reproducible gas chromatography-thermal energy analyzer (g.c.-TEA) method has been developed for the analysis of N-nitrosodiethanolamine (NDELA) in tobacco and tobacco smoke. The extract of tobacco or the trapped particulates of tobacco smoke are chromatographed on silica gel. The NDELA containing fractions are concentrated, silylated and analyzed with a modified g.c.-TEA system. (/sup 14/C)NDELA serves as internal standard for the quantitative analysis. Experimental cigarettes made from tobaccos which were treated with the sucker growth inhibitor maleic hydrazidediethanolamine (MH-DELA) contained 115--420 p.p.b. of NDELA and their smoke contained 20--290 ng/cigarette, whereas hand-suckered tobacco and its smoke were free of NDELA. The tobacco of US smoking products contained 115--420 p.p.b. of NDELA and the mainstream smoke from such products yielded 10--68 ng/cigar or cigarette. NDELA levels in chewing tobacco ranged from 220--280 p.p.b. and in two commercial snuff products were 3,200 and 6,800 p.p.b. Although the five analyzed MH-DELA preparations contained between 0.6--1.9 p.p.m. NDELA it is evident that the major portion of NDELA in tobacco is formed from the DELA residue during the tobacco processing. Based on bioassay data from various laboratories which have shown that NDELA is a relatively strong carcinogen and based on the results of this study the use of MH-DELA for the cultivation of tobacco is questioned.

• Research Organization: Division of Environmental Carcinogenesis, Naylor Dana Institute for Disease Prevention, American Health Foundation, Valhalla, NY
• OSTI ID: 5310902
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 2:11; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AEROSOLS
CARBON 14 COMPOUNDS
CARCINOGENS
CHEMICAL ANALYSIS
CHEMICAL COMPOSITION
CHROMATOGRAPHY
COLLOIDS
DISPERSIONS
GAS CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
QUALITATIVE CHEMICAL ANALYSIS
QUANTITATIVE CHEMICAL ANALYSIS
RESIDUES
SEPARATION PROCESSES
SMOKES
SOLS
TOBACCO PRODUCTS
TOBACCO SMOKES