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Orientation and dynamics of phospholipid head groups in bilayers and membranes determined from /sup 31/P nuclear magnetic resonance chemical shielding tensors

Journal Article · · Biochemistry; (United States)
OSTI ID:7317418
;
/sup 31/P nuclear magnetic resonance (NMR) powder spectra have been used to determine the principal values of the chemical shielding tensors of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidic acid. The shielding tensors in all cases were clearly nonaxial. The principal values for the monoester phosphatidic acid shielding tensor are -40, -4, and 48 ppM relative to 85% H/sub 3/PO/sub 4/. By contrast the diesters have values of -87, -25, and 119 ppM for phosphatidylcholine, -81, -20, and 105 ppM for phosphatidylethanolamine, and -80, -20, and 112 ppM for phosphatidylserine. This difference reflects the sensitivity of the /sup 31/P shielding tensor to chemical environment. Anisotropic motion of the molecules in lamellar dispersions of phospholipids caused an incomplete averaging of the shielding tensors resulting in partially narrowed spectra. Spectra of various phospholipid dispersons were recorded as a function of temperature and transitions observed at the gel-liquid crystalline phase transition temperatures. Using a reasonable set of initial conditions, it was shown that a simple model of molecular motion could successfully predict the observed spectra and their temperature dependences. A preliminary /sup 31/P NMR spectrum of chick embryo fibroblasts is included.
Research Organization:
Univ. of California, Berkeley
OSTI ID:
7317418
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 16:3; ISSN BICHA
Country of Publication:
United States
Language:
English

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104 -- Spectral Procedures-- (-1987)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANISOTROPY
CONNECTIVE TISSUE CELLS
EQUATIONS
ESTERS
FIBROBLASTS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MATHEMATICAL MODELS
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORDER PARAMETERS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORIENTATION
PHOSPHOLIPIDS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
PREDICTION EQUATIONS
RESONANCE
SOMATIC CELLS
STABLE ISOTOPES
TEMPERATURE DEPENDENCE