THE INCORPORATION OF RADIOACTIVE PHOSPHORUS INTO THE PHOSPHOLIPIDS OF HUMAN LEUKEMIC LEUKOCYTES AND PLATELETS

Journal Article · Wed Feb 01 04:00:00 EST 1961 · Journal of Clinical Investigation (U.S.)

DOI:https://doi.org/10.1172/JCI104270· OSTI ID:4084894

Firkin, B G; Williams, W J

The phospholipid composition of platelets and leukocytes from patients with acute and chronic leukemia was studied by a combination of column and paper chromatography. The phospholipid composition of these formed elements was similar and included phosphatidylethanolamine, phosphatidylserine, phosphatidylcholine, sphingomyelin, a phosphoinositide, (probably phosphatidylinositol), and a compoment believed to be phosphatidic acid. The incorporation of P/sup 32/ into the leukocyte and platelet phospholipids of patients given Na/sub 2/-HP/sup 32/O/sub 4/ therapeutically was studied. Radioactivity was present in the phospholipids 18 hr after administration of P/ sup 32/ and was found principally in phosphatidylethanolamine, phospbatidylcholine, and the phosphoinositide. The in vitro incorporation of P/ sup 32/ into the phospholipids of leukemic leukocytes and platelets was also studied. The major incorpcration of radioactivity occurred in the phosphoinositide and a component believed to be phosphatidic acid, in contrast to the pattern of labeling found in vivo. (auth)

• Research Organization: Washington Univ. School of Medicine, St. Louis
• Sponsoring Organization: USDOE
• NSA Number: NSA-15-010628
• OSTI ID: 4084894
• Journal Information: Journal of Clinical Investigation (U.S.), Journal Name: Journal of Clinical Investigation (U.S.) Vol. Vol: 40; ISSN JCINA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
CHROMATOGRAPHY
LEUCOCYTES
LEUKEMIA
LIPIDS
MAN
PHOSPHORUS 32